| 论文题目: | Employing metabolic engineered lipolytic microbial platform for 1-alkene one-step conversion |
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| 作者: | Wang Juli, Yu Haiying, and Zhu Kun* |
| 联系作者: | |
| 刊物名称: | Bioresour Technol |
| 期: | 263 |
| 卷: | |
| 页: | 172-179 |
| 年份: | 2018 |
| 影响因子: | 5.978 |
| 论文下载: | 下载地址 |
| 摘要: | 1-Alkenes are traditionally used as basic chemicals with great importance. Biosynthetic 1-alkenes also have the potential to serve as biofuels. In this study, we engineered a Pseudomonas lipolytic microbial platform for 1-alkene production using hydrophobic substrate as sole carbon source. Fatty acid decarboxylase UndA and UndB were cloned and expressed, which successfully produced 1-alkenes. Optimal culturing temperature and the interruption of competitive pathway were proven to be beneficial to 1-alkene synthesis. Chromosomal integration of UndB conferred 177.8mg/L 1-alkenes (mainly 1-undecene) in lauric acid medium and 128.9mg/L 1-alkenes (mainly 1-pentadecene) in palm oil medium. Thioesterase expression, adjustments of fatty acid degradation pathway and a second copy of UndB improved 1-alkene titer to 1102.6mg/L using lauric acid and 778.4mg/L using palm oil. All in all, this study offers the first demonstration of lipolytic microbial 1-alkene producing platform with highest reported 1-alkene product titer up to date. |
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