| 论文题目: | Detection of glycan-binding proteins using glycan-functionalized quantum dots and gold nanoparticles |
|---|---|
| 作者: | Zhang Zhenxing , Qi Xiaoxiao , Chai Jinfeng , Wu Peixing , Lv Xun*, Cheng Shuihong*, and Li Xuebing* |
| 联系作者: | |
| 刊物名称: | Journal of Carbohydrate Chemistry |
| 期: | 37 |
| 卷: | 4 |
| 页: | 199-209 |
| 年份: | 2018 |
| 影响因子: | 0.983 |
| 论文下载: | 下载地址 |
| 摘要: | Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannosefunctionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs. [GRAPHICS] . |
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