2.

Wei L#, Shu X#, Wang W#, Li F, Fan Y, Li D, Wang R, Liu J, Wang Q, Jiang L, He L, Duan S, Zhao L, Zhang S, Ren J, Zhang Y,Li M*, Feng Y*. (2026) Caspase forms double-stranded helical assembly during the type IV Thoeris antiviral response. Vita. doi: 10.15302/vita.2026.06.0047

3.

Liu Y, Zhao H, Cao X, Jiang S, Fu J, Xue J, Li C, Xu Z*, Li M*, Du W*. (2026) Rapid and quantitative measurement of bacteriophage infectivity via fully automated droplet digital PCR. Nat Commun. 17: 7584. (IF=18.1)

4.Zhao H#, Zhou T#, Zhang M#, Wang C#, Wang R, Shu X, Cheng F, Xue Q, Liu C, Xu J, Cao X, Du J, Wang L, Liu H*, Li M*. (2026) Associate toxin-antitoxin with CRISPR-Cas to harness (ATTACH) engineered microbes. Nucleic Acids Res. 54(5): gkag213. (IF=15.0)

6.Chen Z#, Xue J#, Wang Z, Sun J, Cui Y, Zhu T, Yang H, Li M*, Wu B*. (2025) Small RNA Toxin-Assisted Evolution of GC-Preferred ErCas12a for Enhanced Genome Targeting Range. Advanced Science. e17105. (IF=14.1)

7.Shu X#, Wang R#, Li Z#, Xue Q#, Wang J, Liu J, Cheng F, Liu C, Zhao H, Hu C, Li J*, Ouyang S*, Li M*. (2025) CRISPR-repressed toxin-antitoxin provides herd immunity against anti-CRISPR elements. Nature Chemical Biology. 21(3):337-347.(IF=15.8)

8.Cheng F#, Wu A#, Li Z, Xu J, Cao X, Yu H, Liu Z, Han W, Xiang H*, Li M*. Catalytically active long prokaryotic Argonaute proteins employ PLD nucleases to strengthen immunity against different genetic invaders. (2024) mLife. 3(3):403-416.

9.Li M*#, Gong L#, Cheng F#, Yu H, Zhao D, Wang R, Wang T, Zhang S, Zhou J, Shmakov S, Koonin E, Xiang H*. (2021) Toxin-antitoxin RNA pairs safeguard CRISPR-Cas systems. Science. 372(6541): eabe5601. (IF=56.9)

10.Wang R#, Shu X#, Zhao H#, Xue Q, Liu C, Wu A, Cheng F, Wang L, Zhang Y, Feng J, Wu N, Li M*. (2023) Associate toxin-antitoxin with CRISPR-Cas to kill multidrug-resistant pathogens. Nature Communications. 14(1):2078. (IF=16.6)

11.Liu C#, Wang R#, Li J#, Cheng F#, Shu X#, Zhao H, Xue Q, Yu H, Wu A, Wang L, Hu S, Zhang Y, Yang J, Xiang H*, Li M*. (2023) Widespread RNA-based cas regulation monitors crRNA abundance and anti-CRISPR proteins. Cell Host & Microbe. 31(9):1481-1493. (IF=30.3)

12.Cheng F#, Wu A#, Liu C#, Cao X, Wang R, Shu X, Wang L, Zhang Y, Xiang H*, Li M*. (2022) The toxin-antitoxin RNA guards of CRISPR-Cas evolved high specificity through repeat degeneration. Nucleic Acids Research.50(16):9442-9452. (IF=14.9)

13.Cheng F#, Wang R#, Yu H#, Liu C, Yang J, Xiang H*, Li M*. (2021) Divergent degeneration of creA antitoxin genes from minimal CRISPRs and the convergent strategy of tRNA-sequestering CreT toxins. Nucleic Acids Research. 49(18):10677-10688. (IF=14.9)

14.Gong L, Li M*, Cheng F, Zhao D, Chen Y, Xiang H*. (2019) Primed adaptation tolerates extensive structural and size variations of the CRISPR RNA guide in Haloarcula hispanica. Nucleic Acids Res., 47(11):5880-91. (IF=14.9)

15.Cheng F, Gong L, Zhao D, Yang H, Zhou J, Li M*, Xiang H*. (2017) Harnessing the native type I-B CRISPR-Cas for genome editing in a polyploid archaeon. J. Genet. Genomics., 44(11):541-548. (IF=5.9；封面文章)

代表性图片

CRISPR-Cas和先天免疫系统的协同防御模型

(Nature 657, 226-235, DOI: 10.1038/s41586-026-10833-9)

双RNA型toxin-antitoxin (CreTA)守卫CRISPR免疫

（Science 372, eabe5601, DOI: 10.1126/science.abe5601）

CreR介导Cas效应蛋白的自我调控

(Cell Host & Microbe 31(9):1481-1493, DOI: 10.1016/j.chom.2023.08.005)

申请专利

1.李明，王锐，舒宪，基于特异性核酸酶PSN的无毒筛选标记及其在质粒和噬菌体清除中的应用；申请号：202511175500.3；申请日期：2025.08.21

2.李明，赵会伟，王锐，舒宪，基于CRISPR-Cas 偶联毒素-抗毒素系统构建的条件自杀开关及其应用；申请号：202510216185.8；申请日期：2025.02.26

3.李明，程飞跃，伍蔼慈，李志华，一种新型常温原核 Argonaute 蛋白及其基因和应用；申请号：202311615430.X；申请日期：2023.11.29

4.李明，王锐，舒宪，程飞跃，杨俊，一种偶联的CRISPR和毒素-抗毒素元件及其在消杀耐药细菌中的应用；申请号：202210947272.7；申请日期：2022.08.09

5.向华，杜开心，龚路遥，李明，一种利用I型CRISPR-Cas系统同时实现基因编辑和转录调控的方法；申请号：202011546641.9；申请日期：2020.12.24

6.向华，李明，程飞跃，龚路遥，一种古菌和细菌中通用的RNA型毒素及其相关生物材料；申请号：202011320316.0；申请日期：2020.11.23

7.陈泽华，李明，广谱识别PAM序列的FnCpf1突变体及其应用；授权专利号：CN112111471B；申请日期：2020.09.25