| 论文题目: | Proteomic insights into the temperature responses of a cold-adaptive archaeon Methanolobus psychrophilus R15 |
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| 作者: | Chen Zijuan Feng Deqin, Zhang Bo, Wang Qian, Luo Yuanming, Dong Xiuzhu*. |
| 联系作者: | |
| 刊物名称: | Extremophiles |
| 期: | 19 |
| 卷: | 2 |
| 页: | 249-59 |
| 年份: | 2015 |
| 影响因子: | 2.741 |
| 论文下载: | 下载地址 |
| 摘要: | Cold-adaptive methanogens contribute significantly to methane emission from the cold area, while the cold-adaptive mechanisms used by Archaea remain elusive. Methanolobus psychrophilus R15, a cold-adaptive methanogen isolated from a Tibetan plateau wetland, grows at 0-25 degrees C and optimally at 18 degrees C when isolated; however, it grows optimally at 30 degrees C after culturing at 18 degrees C for several years. Aiming to gain insights into the protein profiles that are involved in optimal growth and cold adaptation of this methanogen, here, we performed a comparative proteomic study using 2D DIGE on the cultures grown at 30, 18 and 4 degrees C. 1439 protein spots (3167 ORFs annotated in the R15 genome) were detected, and 202 of 322 differentially expressed protein spots were identified by MALDI-TOF/TOF. The protein abundance of most enzymes involved in methanogenesis, energy conservation and central metabolism were increased at 30 degrees C, while most ribosome proteins were decreased at 30 degrees C. Proteasome and ROS scavengers increased expressions at 4 degrees C, suggesting more aberrant proteins and ROS formed at lower temperatures. Different from the cold-adaptive Methanococcoides burtonii, some chaperones were increased at 4 degrees C, implying that protein folding was impaired at cold in this psychrophilic archaeon. This study indicates that diverse cold-adaptive mechanisms can be used by different methanogenic Archaea. |
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