Marinobacter halophilus sp. nov., a new halophilic bacterium isolated from a salt lake----中国科学院微生物研究所

科研成果

论文题目:	Marinobacter halophilus sp. nov., a new halophilic bacterium isolated from a salt lake
作者:	Zhong Zhi-Ping, Liu Ying, Liu Hong-Can, Wang Fang, Zhou Yu-Guang, and Liu Zhi-Pei*.
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刊物名称:	Int J Syst Evol Microbiol
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年份:	2015
影响因子:	2.632
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摘要:	A Gram-staining-negative bacterium, strain XCD-X12T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12T were non-spore-forming rods, 0.4-0.7 microm wide, 2.1-3.2 microm long and motile with a single polar flagellum. Strain XCD-X12T was strictly aerobic, catalase- and oxidase-positive. Growth was observed in the presence of 0-20.0 % (w/v) NaCl (optimum, 4.0-8.0 %), and at 4-35 degrees C (optimum, 30 degrees C) and pH 6.5-10.5 (optimum, pH 8.5). It contained Q-9 as predominant respiratory quinone. The major fatty acids (>10.0 %) were C16:0, C16:1omega9c and C18:1omega9c. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidglycerol (DPG), two unknown phospholipids and an uncharacterized amino-phospholipid. The DNA G+C content was 55.6 mol % (Tm). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12T was associated with the genus Marinobacter, and showed highest 16S rRNA gene sequence similarity to M. hydrocarbonoclasticus ATCC 49840T (97.4 %), M. vinifirmus FB1T (96.8 %), M. excellens KMM 3809T (96.8 %) and M. antarcticus ZS2-30T (96.7 %). DNA-DNA relatedness of strain XCD-X12T to M. hydrocarbonoclasticus CGMCC 1.7683T was 34 +/- 5 %. Based on the data, it is concluded that strain XCD-X12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halophilus sp. nov. is proposed. The type strain is XCD-X12T (=CGMCC 1.12481T= JCM 30472T).