叶健研究组

虫媒病毒与宿主互作 研究组

组长:叶健 博士 研究员 博士生导师

研究方向
1. 虫媒病毒自然宿主的免疫适应;2. 植物抵御虫媒病毒免疫网络解析

 

研究内容及意义

病毒的自然疫源圈与人类社会文明圈的交汇导致新发、突发、再发传染病流行爆发。媒介节肢动物(简称虫媒)通过刺吸和吸血等取食过程从感染宿主获得病毒并将其传播给健康宿主,虫媒本身通常能耐受病毒,而受到感染的宿主(人、动物或植物)却引起疾病或病害。自然条件下病毒借助虫媒传播,不但涉及病毒本身,而且涉及宿主、媒介以及宿主和媒介体内的共生微生物,是一个多因素的复杂生物学过程。目前,世界范围内对虫媒这一类自然宿主生物传播病毒的生物学基础和分子生物学机制的认识依然不足。多数虫媒病毒病至今缺乏像临床药物和疫苗等在内的有效控制手段,在人及动植物领域,对于新发突发虫媒病毒病均只能在暴发后采取被动控制措施。因此,通过多学科交叉合作,系统深入了解虫媒传播病毒生命循环的基本过程和机制迫在眉睫,对虫媒病毒传播的生物学基础和病毒适应跨界宿主机制进行解析,将防控虫媒病毒病的关口前移,研发抗病毒手段势在必行。本研究组在国家自然基金委重大、重点、科技部重点研发及中科院先导等项目大力支持下主要开展以下两个研究方向的工作:


1. 虫媒病毒自然宿主的免疫适应。许多虫媒病毒,进化获得操纵媒介生物行为的能力,加速病毒自身向脊椎动物,鸟类,昆虫和植物的传播。这种病毒与昆虫媒介和谐共处,并通过操纵宿主有利于媒介昆虫,从而间接有利于病毒传播的行为,被学界称为病毒的高“情商,EQ”。这种操纵行为(Host manipulation)极大的促进了病毒的流行爆发。我们的前期研究发现了宏观生态学效应的分子机制,即病毒对宿主的代谢重塑改变了昆虫—植物的化学通讯。我们致力于将微观分子生物学、遗传学、媒介昆虫神经生物学技术,发掘、解析和利用抗虫媒病毒相关的代谢重塑机制,发展新型广谱抗病毒化合物。本课题组最近发现入侵双生病毒具有的全新生态学功能,双生病毒感染棉花等植物不仅促进媒介昆虫烟粉虱,而且阻止非介体竞争对手棉铃虫和蚜虫的生态功能,促进双生病毒入侵危害。具体研究成果参见近期代表工作1和近期代表工作2。2. 植物抵御虫媒病毒免疫网络解析。最经济有效的防控虫媒植物病毒病的方法是获得抗性品种,解析宿主的抗性机制至关重要。通常实验研究在实验室条件下,植物对一种抗性(病毒或者昆虫)机制,这种研究往往不符合自然条件的病毒传播规律,因为80%的植物病毒在自然条件下由媒介昆虫传播,而作物往往受到虫媒外加病毒的双重复杂胁迫。我们研究植物应对复杂生物胁迫的分子、细胞机制,并通过先进生物技术手段发展抗虫媒病毒传播和传播的新方法、新种质材料,有效防控新发病毒疾病提供了理论基础和绿色应用生物技术,获省部级科技进步奖一项(参见近期代表工作3)

 

研究组长

叶健 博士、研究员、农业室副主任、国家杰出青年科学基金获得者(2021)、
国家优秀青年科学基金获得者(2015)

电话:64807363O);64806195L
电子邮件:jianye#im.ac.cn
(请将#换成@
通讯地址:北京市朝阳区北辰西路1号院3号中科院微生物所
邮政编码:100101

 

学习经历
2000–2007,中国科学院微生物研究所,博士
1996–2000,浙江大学农业与生物技术学院,学士

 

工作经历
2019-,        农业微生物与生物技术研究室,副主任

2017–,       中科院微生物所,课题组长 
2014–2017,中科院微生物所,青年课题组长
2013–2014,新加坡国立大学淡马锡生命科学研究院,
Principal Investigator
2011–2013,新加坡国立大学淡马锡生命科学研究院,
Biotechnology Manager
2009–2010,新加坡国立大学淡马锡生命科学研究院,
Research Fellow
2007–2009,新加坡国立大学淡马锡生命科学研究院,博士后

 

获奖
浙江大学 竺可桢奖学金

研究队伍(请将#换成@

工作人员和特别研究助理
赵平芝 女 副研究员         zhaopz#im.ac.cn
张    璇 女    助理研究员     zhangx#im.ac.cn
孙艳伟 女 助理研究员 
sunyw#im.ac.cn
王    宁 女    特别研究助理  wangning0847#163.com
李玉林 男 特别研究助理 lyl6923#163.com

在读研究生
直博生    吴秀娟    女    wuxj#im.ac.cn
博士生    黄显德    男    hxd199287#163.com
博士生    徐 爽    男   
979138774#qq.com
博士生    王    端    女    781988712#qq.com
博士生    梁    琳    女    lianglinbrave#163.com
博士生    高凯星    男    gaokaixing21#mails.ucas.ac.cn
硕士生    李奉昶    男    1063493171#qq.com
硕士生    王宏伟    男    hongweiwangbio#163.com
硕士生    高连博    男    861090452#qq.com
硕士生    吴金宝    男    15070061073#163.com
硕士生    尹策策    男     15605196332#163.com
硕士生    杨     欢   女     2609286621#qq.com

 

著书章节(*通讯作者, #共同第一作者)

  1. Ma YH, Ye J*. 2017. Lipid biosynthesis and regulation in Jatropha curcas, an emerging model for woody energy plants. The Jatropha Genome. Compendium of Plant Genomes. Suguru Tsuchimoto (Ed.) Jatropha. Springer Science Publishers, New York.

  2. Ye J, Wang CM, Yue GH. 2017. Jatropha breeding. The Jatropha Genome. Compendium of Plant Genomes. Suguru Tsuchimoto (Ed.) Jatropha. Springer Science Publishers, New York.

  3. Ye J*, Hong Y, Qu J, Wang CM. 2012. Improvement of Jatropha oil by genetic transformation. Jatropha, Challenges for a New Energy Crop-Genetic Improvement and Biotechnology, vol. 2. Springer Science Publishers, New York.

  4. Qu J, Ye J, Fang RX. 2012. Artificial MicroRNAs for Plant Virus Resistance, Antiviral Resistance in Plants: Methods and Protocols, Methods in Molecular Biology, vol. 894

 

发表论文(*通讯作者, #共同第一作者)

 

2022年

  1. Chen GM#, Xuan W#, Zhao PZ#, Yao XM, Peng C, Tian YL, Ye J, Wang BX, He J, Chi WC, Yu J, Ge YW, Li J, Dai ZY, Xu DY, Wang CM, Wan JM. OsTUB1 confers salt-insensitivity by interacting with Kinesin13A to stabilize microtubule and ion transporter in rice. 2022. New Phytologist. https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.18282
  2. Liu XL, Tian YL, Chi WC, Zhang HZ, Yu J, Chen GM, Wu W, Jiang XZ, Wang SS, Lin ZX, Xuan W, Ye J, Wang BX, Liu Y, Sun ZG, Xu DY, Wang CM, Wan JM. Alternative splicing of OsGS1;1 affects nitrogen-use efficiency, grain development, and amylose content in rice. 2022. The Plant Journal. https://doi.org/10.1111/tpj.15768
  3. Wang D, Bishnu Dawadi, Qu J, Ye J*, Light-Engineering Technology for Enhancing Plant Disease Resistance. Front. Plant Sci. 2022. 12:805614
  4. Wang D, Zhang X, Chen DX, Ye J*. First report of Sida leaf curl virus and associated betasatellite in tobacco. Plant Disease. 2022. 106: 1078

 

2021年

  1. Zhao PZ, Zhang X, Gong YQ, Wang D, Xu DQ, Wang N, Sun YW, Gao LB, Liu SS, Deng XW, Kliebenstein DJ, Zhou XP, Fang RX, and Ye J* Red-light is an environmental effector for mutualism between begomovirus and its vector whitefly. PLoS Pathogens. 2021. 10.1371/journal.ppat.1008770
  2. Zhao DN, Li YL, Song Y, Yang BJ, Ding X, Gao, F, Ye J, Hao XJ, Zhang Y, Li SL. Ten new nortriterpenes from Euphorbia resinifera and their anti-tomato yellow leaf curl virus activities. Fitoterapia. 2021. 153. 104989
  3. Ye J*, Zhang LL, Zhang X, Wu XJ, Fang RX*. Plant defense networks against insect-borne pathogens. Trends in Plant Science. 2021. 26 (3) 272-287

 

2020年

  1. Li YL, Zhang Y, Zhao PZ, Hu ZX, Gu YC, Ye J*, Hao XJ*. Two new steroidal alkaloids from the rhizomes of Veratrum nigrum L. and their anti-TYLCV activity. Fitoterapia. 2020. 147. 104731

  2. Wu XJ, Ye J*.  Manipulation of Jasmonate Signaling by Plant Viruses and Their Insect Vectors. Viruses. 2020. 12(2), 148.

  3. 王端,叶健*. 植物病毒资源属性和应用基础研究进展. 生物资源. 2020. 42(1)1-8

  4. Wang D, Zhang X, Yao XM, Zhang P, Fang RX, Ye J*.  A 7-Amino-Acid Motif of Rep protein essential for virulence is critical for triggering host defense against Sri Lankan cassava mosaic virus. Molecular Plant-Microbe Interactions, 2020. Jan;33(1):78-86 DOI: .1094/MPMI-06-19-0163-FI. 
     

2019年 

  1. Zhao PZ, Yao XM, Cai CX, Li R, Du J, Sun YW, Wang MY, Zou Z, Wang QM, Kliebenstein DJ , Liu SS, Fang RX and Ye J*. Viruses mobilize plant immunity to deter non-vector insect herbivores. Science Advances. DOI: 10.1126/sciadv.aav9801.

  2. Wu XJ#, Xu S#, Zhao PZ, Zhang X, Yao XM, Sun YW,Fang RX, Ye J*. The Orthotospovirus nonstructural protein NSs suppresses plant MYC-regulated jasmonate signaling leading to enhanced vector attraction and performance. PLoS Pathogens. DOI:10.1371/journal.ppat.1007897.

  3. Wang N, Zhao PZ, Ma YH, Yao XM, Sun YW, Huang XD, Jin JJ, Zhang YJ, Zhu CX, Fang RX, Ye  J*, A whitefly effector Bsp9 targets host immunity regulator WRKY33 to promote performance. Philos. Trans. Roy. Soc. B, 2019. 374: 20180313. 

  4.  

    Tang WJ#, Ye J#, Yao XM#, Zhao PZ, Xuan W, Tian YL, Zhang YY, Xu S, An HZ, Chen GM, Yu J, Wu W, Ge YW, Liu XL, Li J, Zhang HZ, Zhao YQ, Yang B, Jiang XZ, Peng C, Zhou C, Terzaghi W, Wang CM* & Wan JM* Genome-wide associated study identified NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice. Nature Communications. 2019. 10(1):5279

 

2018年

  1. Wang D, Yao, XM, Huang GX, Shi T, Wang GF, Ye J*, First report of Sri Lankan cassava mosaic virus infected cassava in China, Plant Disease 2018, DOI: 10.1094/PDIS-09-18-1590-PDN 

  2. Ye J*, Wang CM, Sun YW, Qu J, Mao HZ, Chua NH*. 2018. Overexpression of  JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Frontiers in Plant Sciences, 2018. fpls.2018.01479 

     

2017

  1. 叶 健*,龚雨晴,方荣祥*,病毒—媒介昆虫—植物三者互作研究进展及展望. 中国科学院院刊. 已接收

  2. Sun YW#, Wang CM#, Wang N#, Jiang XY, Mao HZ, Zhu CX, Wen FJ, Wang XH, Lu ZJ, Yue GH, Xu ZF, Ye J*. 2017. Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas. Scientific reports. 7: 40844.

  3. Jin JJ, Sun YW#, Qu J, Rahmadsyah, Lim CH, Alfiko Y, NurEstyaBte Rahman, Suwanto A, Yue GH, Wong LS, Chua NH, Ye J*. 2017. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Scientific reports. 7: 439.

 

2016

  1. Jun Liu#, Shulin Deng#, HW Wu#, Ye J, Hui-Wen Wu, Hai-Xi Sun, and Nam-Hai Chua. 2016. CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis in Arabidopsis. Plant physiology. 17 (1) 424-436.

  2. Tang W#, Wu T#, Ye J, Sun J, Jiang Y, Yu J, Tang J, Chen G, Wang C, Wan J. 2016. SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice. BMC Plant Biology 16:93.

  3. Wang N#, Yao YM#, Zhu CX, Wen FJ*, Ye J*. 2016 Towards sustainable protection against insect-borne plant viral diseases: phytohormones and beyond. Science Foundation in China. 24(2). Review. Cover

  4. Pingzhi Zhao, Lubomir N. Sokolov, Ye Jian, Cheng-Yi Tang, Jisen Shi, Yan Zhen, Wenzhi Lan, Zhi Hong, Jinliang Qi, Gui-Hua Lu, Girdhar K. Pandey & Yong-Hua Yang. 2016 The LIKE SEX FOUR2 regulates root development by modulating reactive oxygen species homeostasis in Arabidopsis. Scientific reports. srep28683.

  5. Jin JJ#, Lee M#, Bai B#, Sun Yanwei#, Qu J, Rahmadsyah, Yuzer A, Lim CH, Suwanto A, Sugiharti M, Wong LS, Ye Jian*, Chua NH* and Yue GH*. 2016 Draft genome sequence of an elite Dura palm and whole-genome patterns of DNA variation in oil palm. DNA Research. 23:527.

  6. 龚雨晴,姚香梅,徐爽,孙艳伟,王宁,马永焕,赵平芝,应晓宝,叶健*. 微生物组学研究与经济作物虫传病害的绿色防控. 《植物保护与农产品安全》 201612

 

2015

  1. Ye J*, Yang J, Sun Y, Zhao P, Gao S, Jung C, Qu J, Fang R, Chua NH*. 2015. Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis. PLoS pathogens 11:e1005196.

  2. Sun YW#, Tee CS#, Ma YH#, Wang G, Yao XM, Ye J*. 2015. Attenuation of Histone Methyltransferase KRYPTONITE-mediated transcriptional gene silencing by Geminivirus. Scientific reports 5:16476.

  3. Li R, Tee C, Jiang Y, Jiang X, Venkatesh P, Sarojam R, Ye J*. 2015. A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X. Scientific reports 5:9682.

  4. Wang H NQ, Wu HW, Liu J, Ye J, Yu N, Chua NH. 2015. Analysis of noncoding transcriptome in rice and maize uncovers roles of conserved lncRNAs associated with agriculture traits. The Plant Journal 84 2: 404-416.

  5. Lee M, Xia J, Zou Z, Ye J, Rahmadsyah, Alfiko Y, Jin J, Lieando J, Purnamasari M, Lim C, Suwanto A, Wong L, Chua N, Yue G. 2015. A consensus linkage map of oil palm and a major QTL for stem height. Scientific reports 5:8232.

 

2014

  1. Li R, Weldegergis BT, Li J, Jung C, Qu J, Sun Y, Qian H, Tee C, van Loon JJ, Dicke M, Chua NH, Liu SS, Ye J*. 2014. Virulence factors of geminivirus interact with MYC2 to subvert plant resistance and promote vector performance. The Plant cell 26:4991-5008.

  2. Ye J, Qu J, Mao HZ, Ma ZG, Rahman NE, Bai C, Chen W, Jiang SY, Ramachandran S, Chua NH. 2014. Engineering geminivirus resistance in Jatropha curcus. Biotechnology for biofuels 7:149.

  3. Ye J, Geng Y, Zhang B, Mao H, Qu J, Chua N. 2014. The Jatropha FT ortholog is a systemic signal regulating growth and flowering time. Biotechnology for biofuels 7:19.

  4. Ye J, Liu P, Zhu C, Qu J, Wang X, Sun Y, Sun F, Jiang Y, Yue G, Wang C. 2014. Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha. Functional & integrative genomics 14:757-766.

  5. Wang G, Sun Y, Xu R, Qu J, Tee C, Jiang X, Ye J*. 2014. DNA-A of a highly pathogenic Indian cassava mosaic virus isolated from Jatropha curcas causes symptoms in Nicotiana benthamiana. Virus genes 48:402-405.

 

2013年及以前

  1. Wang C, Ye J, Tang W, Liu Z, Zhu C, Wang M, Wan J. 2013. Loop nucleotide polymorphism in a putative miRNA precursor associated with seed length in rice (Oryza sativa L.). International journal of biological sciences 9:578-586.

  2. Wang CM, Liu P, Sun F, Li L, Liu P, Ye J, Yue GH. 2012. Isolation and identification of miRNAs in Jatropha curcas. International journal of biological sciences 8:418-429.

  3. Qu J, Ye J#, Geng YF, Sun YW, Gao SQ, Zhang BP, Chen W, Chua NH. 2012. Dissecting functions of KATANIN and WRINKLED1 in cotton fiber development by virus-induced gene silencing. Plant physiology 160:738-748.

  4. Ye J*, Hong Y, Qu J, Wang C. 2012. Improvement of Jatropha oil by genetic transformation. Jatropha, Challenges for a New Energy Crop- Genetic Improvement and Biotechnology, vol. 2. Springer Science Publishers, New York.

  5. Qu J, Mao HZ, Chen W, Gao SQ, Bai YN, Sun YW, Geng YF, Ye J*. 2012. Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. Biotechnology for biofuels 5:10.

  6. Qu J, Ye J, Fang R. 2012. Artificial MicroRNAs for Plant Virus Resistance, Antiviral Resistance in Plants: Methods and Protocols, Methods in Molecular Biology, vol. 894.

  7. Sun F, Liu P, Ye J, Lo LC, Cao S, Li L, Yue GH, Wang CM. 2012. An approach for jatropha improvement using pleiotropic QTLs regulating plant growth and seed yield. Biotechnology for biofuels 5:42.

  8. Gao S, Qu J, Chua NH, Ye J*. 2010. A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. Archives of virology 155:607-612.

  9. Ye J, Qu J, Zhang JF, Geng YF, Fang RX. 2009. A critical domain of the Cucumber mosaic virus 2b protein for RNA silencing suppressor activity. FEBS letters 583:101-106.

  10. Ye J, Qu J, Bui HT, Chua NH. 2009. Rapid analysis of Jatropha curcas gene functions by virus-induced gene silencing. Plant biotechnology journal 7:964-976.

  11. Qu J, Ye J#, Fang R. 2007. Artificial microRNA-mediated virus resistance in plants. Journal of virology 81:6690-6699.

  12. Xu Y, Ye J#, Liu H, Cheng E, Yang Y, Wang W, Zhao M, Zhou D, Liu D, Fang R. 2008. DNA-templated CMV viral capsid proteins assemble into nanotubes. Chemical Communications 1:49-51.

  13. Ye J, Liu L, Xue Y, Qu J, Gao G, Fang R. 2007. Efficient depletion of multiple SARS-CoV mRNAs by a single small interfering RNA targeting the leader sequence. Progress in Biochemistry and Biophysics 34:1092-1100.

 

国际专利

  1. Jian Ye, et al. Transcriptome and functional analysis reveals a single gene hybrid vigor for oil yield in oil palm. (US Provisional Patent Application No. 62/082,341)

  2. Jian Ye et al.. A method to enhance plant virus resistance. (United States patent, PCT/SG2014/00003)

  3. Jian Ye et al. Control of pests in plants. (U.S. Patent Application US 2012/02400288A1).

  4. Jian Ye et al. Virus induced gene silencing (VIGS) for functional analysis of genes in cotton. (United States Patent 20120096595).

  5. Jian Ye et al. Functional analysis of Jatropha curcas genes. (United States Patent 20110265233).

  6. Jian Ye et al. Genetic Transformation of Jatropha curcas. (United States Patent 20110247099 A1).

  7. Jian Ye et al. Increasing cotton fiber length by manipulating transcription factor. (United States Patent PCT/SG2012/000139).

  8. Jian Ye et al. Microsomal ω6 Oleate Desaturases. (U.S. patent application PCT/SG2011/000337).

 

近期代表工作1双生病毒抑制植物MYC2 介导的茉莉酸(JA)抗虫代谢途径,同介体昆虫烟粉虱形成互惠共生关系。A.植物MYC2转录因子转录激活抗虫次级代谢物合成; B. 双生病毒抑制植物MYC2介导的抗虫反应; C. 双生病毒同烟粉虱形成依赖于寄主植物的互惠共生关系 (Li et al. Plant Cell 2014)

近期代表工作2双生病毒“劫持”细胞质RNA降解途径抑制植物基因沉默并促进病毒复制。Ye et al. PLoS Pathogens 2015

近期代表工作3:棉花曲叶病毒感染增强棉花对棉铃虫的抗性: A. 烟粉虱和棉铃虫在健康棉花和感染棉花曲叶病毒棉花上存活五天后的照片;B. 烟粉虱在健康棉花和感染棉花曲叶病毒棉花上的存活数;C. 棉铃虫在健康棉花、感染棉花曲叶病毒棉花和转基因Bt棉花上体重增长情况。

 

 

 

 

 

 

 

 

近80%的植物病毒病害由昆虫媒介传播,1100种已知植物病毒中30%由烟粉虱传播。我们主要研究在自然条件下病毒病害发生的多元生物互作体系的贡献及其互作机制,探求病毒病害防治的重要节点,结合利用大数据平台技术,设计农业生产条件下有效的病毒防治策略。