叶健研究组

虫媒病毒与宿主互作 研究组

组长:叶健 博士 研究员

研究方向
1. 虫媒病毒—植物—介体昆虫互作的分子机理;2. 重大虫媒病毒入侵机制及流行预警


研究内容及意义

1. 虫媒传染病,包括以蜱类、蚊虫、蚤类、螨、蠓、蚋和白蛉等吸血性节肢动物媒介传播的病原微生物引起人类和动物疾病,以及以粉虱、蓟马、叶蝉与飞虱等媒介昆虫以刺吸植物汁液方式传播的疾病。媒介生物携带病原微生物种类繁多,而且与人类活动密切的鼠与蝙蝠、可远距离传播病原体的候鸟等脊椎动物病媒,虫媒疾病跨境传播普遍,防控难度大,给公共卫生领域和农业生产带来巨大挑战。近年来我国几乎每年都出现新发虫媒传染病,应对形势严峻。本课题组致力于将微观分子生物学、遗传学、媒介昆虫神经生物学技术,整合宏观生物学的现象和规律,寻找病毒病害流行爆发的宏观生态学现象背后的遗传学和生物化学机制,努力建立复杂生态系统多元互作分子机制提供数学模型,并利用自然规律设计人工干扰技术,发展新型广谱抗病毒化合物。具体研究成果参见近期代表工作1和近期代表工作2。

2. 在农业虫媒病害领域,国外重大虫媒传染病输入风险不断,例如烟粉虱传双生病毒和蓟马传植物布尼亚病毒,具有农业生物安全风险。虫媒病毒病传播迅速,危害严重。本课题组最近发现入侵双生病毒具有的全新生态学功能,双生病毒感染棉花等植物不仅促进媒介昆虫烟粉虱,而且阻止非介体竞争对手棉铃虫和蚜虫的生态功能,促进双生病毒入侵危害。这些工作为指导有效预警新发病毒疾病提供了理论基础(参见近期代表工作3)

研究组长

叶健 博士、研究员、农业室副主任、国家优秀青年科学基金获得者(2015

电话:64807363O);64806195L
电子邮件:jianye#im.ac.cn(请将#换成@
通讯地址:北京市朝阳区北辰西路1号院3号中科院微生物所
邮政编码:100101


学习经历
2000–2007,中国科学院微生物研究所,博士
1996–2000,浙江大学农业与生物技术学院,学士


工作经历
2019-,        农业微生物与生物技术研究室,副主任

2017–,       中科院微生物所,课题组长 
2014–2017,中科院微生物所,青年课题组长
2013–2014,新加坡国立大学淡马锡生命科学研究院,
Principal Investigator
2011–2013,新加坡国立大学淡马锡生命科学研究院,
Biotechnology Manager
2009–2010,新加坡国立大学淡马锡生命科学研究院,
Research Fellow
2007–2009,新加坡国立大学淡马锡生命科学研究院,博士后


获奖
浙江大学 竺可桢奖学金

研究队伍(请将#换成@

工作人员
孙艳伟 女 助理研究员 
sunyw#im.ac.cn
姚香梅 女 助理研究员 yaoxm#im.ac.cn
赵平芝 女 助理研究员    zhaopz#im.ac.cn
尹策策    男    科研助理       15605196332#163.com

在站博士后

王 宁   女    wangning0847#163.com
张 璇 女 zhangxuan1207#cau.edu.cn

在读研究生
直博生    吴秀娟    女    wuxj#im.ac.cn
博士生    黄显德    男    hxd199287#163.com
博士生    徐 爽    男    979138774#qq.com
博士生    王    端      女    781988712#qq.com
博士生(客座)李玉林    男     lyl6923#163.com
硕士生    张竟引    女    jingyinchang#163.com
硕士生    李奉昶    男    1063493171#qq.com
硕士生    王宏伟    男    hongweiwangbio#163.com  

已毕业研究生
硕士生    马永焕    女    18766177310#163.com
硕士生    龚雨晴    女    gongyuqing15#mails.ucas.ac.cn
硕士生    徐    爽    男    979138774#qq.com
硕士生    王    端    女    781988712#qq.com

已毕业客座研究生
博士生    王 宁    女    山东农业大学    wangning0847#163.com

本科实习生
吴晓娟(山东农业大学)
王端(河北大学)
黄昭(北京农学院)
赵启祥(北京农学院)
王世洋(河北大学)
李苗苗(西南大学)

张莹紫(北京农学院) 
古丽(北京农学院) 
王祁靖 (大连理工大学)


著书章节(*通讯作者, #共同第一作者)

  1. Ma YH, Ye J*. 2017. Lipid biosynthesis and regulation in Jatropha curcas, an emerging model for woody energy plants. The Jatropha Genome. Compendium of Plant Genomes. Suguru Tsuchimoto (Ed.) Jatropha. Springer Science Publishers, New York.

  2. Ye J, Wang CM, Yue GH. 2017. Jatropha breeding. The Jatropha Genome. Compendium of Plant Genomes. Suguru Tsuchimoto (Ed.) Jatropha. Springer Science Publishers, New York.

  3. Ye J*, Hong Y, Qu J, Wang CM. 2012. Improvement of Jatropha oil by genetic transformation. Jatropha, Challenges for a New Energy Crop-Genetic Improvement and Biotechnology, vol. 2. Springer Science Publishers, New York.

  4. Qu J, Ye J, Fang RX. 2012. Artificial MicroRNAs for Plant Virus Resistance, Antiviral Resistance in Plants: Methods and Protocols, Methods in Molecular Biology, vol. 894


发表论文(*通讯作者, #共同第一作者)

 

2020年

  1. Wu XJ, Ye J*.  Manipulation of Jasmonate Signaling by Plant Viruses and Their Insect Vectors. Viruses. 2020. 12(2), 148.

  2. 王端, 叶健*. 植物病毒资源属性和应用基础研究进展. 生物资源. 2020. 42(1)1-8

  3. Wang D, Zhang X, Yao XM, Zhang P, Fang RX, Ye J*.  A 7-Amino-Acid Motif of Rep protein essential for virulence is critical for triggering host defense against Sri Lankan cassava mosaic virus. Molecular Plant-Microbe Interactions, 2020. Jan;33(1):78-86 DOI: .1094/MPMI-06-19-0163-FI. 
     

2019年 

  1. Zhao PZ, Yao XM, Cai CX, Li R, Du J, Sun YW, Wang MY, Zou Z, Wang QM, Kliebenstein DJ , Liu SS, Fang RX and Ye J*. Viruses mobilize plant immunity to deter non-vector insect herbivores. Science Advances. DOI: 10.1126/sciadv.aav9801.

  2. Wu XJ#, Xu S#, Zhao PZ, Zhang X, Yao XM, Sun YW,Fang RX, Ye J*. The Orthotospovirus nonstructural protein NSs suppresses plant MYC-regulated jasmonate signaling leading to enhanced vector attraction and performance. PLoS Pathogens. DOI:10.1371/journal.ppat.1007897.

  3. Wang N, Zhao PZ, Ma YH, Yao XM, Sun YW, Huang XD, Jin JJ, Zhang YJ, Zhu CX, Fang RX, Ye  J*, A whitefly effector Bsp9 targets host immunity regulator WRKY33 to promote performance. Philos. Trans. Roy. Soc. B, 2019. 374: 20180313. 

  4. Tang WJ#, Ye J#, Yao XM#, Zhao PZ, Xuan W, Tian YL, Zhang YY, Xu S, An HZ, Chen GM, Yu J, Wu W, Ge YW, Liu XL, Li J, Zhang HZ, Zhao YQ, Yang B, Jiang XZ, Peng C, Zhou C, Terzaghi W, Wang CM* & Wan JM* Genome-wide associated study identified NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice. Nature Communications. 2019. 10(1):5279

 

 

2018年

  1. Wang D, Yao, XM, Huang GX, Shi T, Wang GF, Ye J*, First report of Sri Lankan cassava mosaic virus infected cassava in China, Plant Disease 2018, DOI: 10.1094/PDIS-09-18-1590-PDN 

  2. Ye J*, Wang CM, Sun YW, Qu J, Mao HZ, Chua NH*. 2018. Overexpression of  JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Frontiers in Plant Sciences, 2018. fpls.2018.01479 

     

 

2017

  1. 叶 健*,龚雨晴,方荣祥*,病毒—媒介昆虫—植物三者互作研究进展及展望. 中国科学院院刊.已接收

  2. Sun YW#, Wang CM#, Wang N#, Jiang XY, Mao HZ, Zhu CX, Wen FJ, Wang XH, Lu ZJ, Yue GH, Xu ZF, Ye J*. 2017. Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas. Scientific reports. 7: 40844.

  3. Jin JJ, Sun YW#, Qu J, Rahmadsyah, Lim CH, Alfiko Y, NurEstyaBte Rahman, Suwanto A, Yue GH, Wong LS, Chua NH, Ye J*. 2017. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Scientific reports. 7: 439.



2016

  1. Jun Liu#, Shulin Deng#, HW Wu#, Ye J, Hui-Wen Wu, Hai-Xi Sun, and Nam-Hai Chua. 2016. CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis in Arabidopsis. Plant physiology. 17 (1) 424-436.

  2. Tang W#, Wu T#, Ye J, Sun J, Jiang Y, Yu J, Tang J, Chen G, Wang C, Wan J. 2016. SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice. BMC Plant Biology 16:93.

  3. Wang N#, Yao YM#, Zhu CX, Wen FJ*, Ye J*. 2016 Towards sustainable protection against insect-borne plant viral diseases: phytohormones and beyond. Science Foundation in China. 24(2). Review. Cover

  4. Pingzhi Zhao, Lubomir N. Sokolov, Ye Jian, Cheng-Yi Tang, Jisen Shi, Yan Zhen, Wenzhi Lan, Zhi Hong, Jinliang Qi, Gui-Hua Lu, Girdhar K. Pandey & Yong-Hua Yang. 2016 The LIKE SEX FOUR2 regulates root development by modulating reactive oxygen species homeostasis in Arabidopsis. Scientific reports. srep28683.

  5. Jin JJ#, Lee M#, Bai B#, Sun Yanwei#, Qu J, Rahmadsyah, Yuzer A, Lim CH, Suwanto A, Sugiharti M, Wong LS, Ye Jian*, Chua NH* and Yue GH*. 2016 Draft genome sequence of an elite Dura palm and whole-genome patterns of DNA variation in oil palm. DNA Research. 23:527.

  6. 龚雨晴,姚香梅,徐爽,孙艳伟,王宁,马永焕,赵平芝,应晓宝,叶健*. 微生物组学研究与经济作物虫传病害的绿色防控. 《植物保护与农产品安全》 201612



2015

  1. Ye J*, Yang J, Sun Y, Zhao P, Gao S, Jung C, Qu J, Fang R, Chua NH*. 2015. Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis. PLoS pathogens 11:e1005196.

  2. Sun YW#, Tee CS#, Ma YH#, Wang G, Yao XM, Ye J*. 2015. Attenuation of Histone Methyltransferase KRYPTONITE-mediated transcriptional gene silencing by Geminivirus. Scientific reports 5:16476.

  3. Li R, Tee C, Jiang Y, Jiang X, Venkatesh P, Sarojam R, Ye J*. 2015. A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X. Scientific reports 5:9682.

  4. Wang H NQ, Wu HW, Liu J, Ye J, Yu N, Chua NH. 2015. Analysis of noncoding transcriptome in rice and maize uncovers roles of conserved lncRNAs associated with agriculture traits. The Plant Journal 84 2: 404-416.

  5. Lee M, Xia J, Zou Z, Ye J, Rahmadsyah, Alfiko Y, Jin J, Lieando J, Purnamasari M, Lim C, Suwanto A, Wong L, Chua N, Yue G. 2015. A consensus linkage map of oil palm and a major QTL for stem height. Scientific reports 5:8232.



2014

  1. Li R, Weldegergis BT, Li J, Jung C, Qu J, Sun Y, Qian H, Tee C, van Loon JJ, Dicke M, Chua NH, Liu SS, Ye J*. 2014. Virulence factors of geminivirus interact with MYC2 to subvert plant resistance and promote vector performance. The Plant cell 26:4991-5008.

  2. Ye J, Qu J, Mao HZ, Ma ZG, Rahman NE, Bai C, Chen W, Jiang SY, Ramachandran S, Chua NH. 2014. Engineering geminivirus resistance in Jatropha curcus. Biotechnology for biofuels 7:149.

  3. Ye J, Geng Y, Zhang B, Mao H, Qu J, Chua N. 2014. The Jatropha FT ortholog is a systemic signal regulating growth and flowering time. Biotechnology for biofuels 7:19.

  4. Ye J, Liu P, Zhu C, Qu J, Wang X, Sun Y, Sun F, Jiang Y, Yue G, Wang C. 2014. Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha. Functional & integrative genomics 14:757-766.

  5. Wang G, Sun Y, Xu R, Qu J, Tee C, Jiang X, Ye J*. 2014. DNA-A of a highly pathogenic Indian cassava mosaic virus isolated from Jatropha curcas causes symptoms in Nicotiana benthamiana. Virus genes 48:402-405.



2013年及以前

  1. Wang C, Ye J, Tang W, Liu Z, Zhu C, Wang M, Wan J. 2013. Loop nucleotide polymorphism in a putative miRNA precursor associated with seed length in rice (Oryza sativa L.). International journal of biological sciences 9:578-586.

  2. Wang CM, Liu P, Sun F, Li L, Liu P, Ye J, Yue GH. 2012. Isolation and identification of miRNAs in Jatropha curcas. International journal of biological sciences 8:418-429.

  3. Qu J, Ye J#, Geng YF, Sun YW, Gao SQ, Zhang BP, Chen W, Chua NH. 2012. Dissecting functions of KATANIN and WRINKLED1 in cotton fiber development by virus-induced gene silencing. Plant physiology 160:738-748.

  4. Ye J*, Hong Y, Qu J, Wang C. 2012. Improvement of Jatropha oil by genetic transformation. Jatropha, Challenges for a New Energy Crop- Genetic Improvement and Biotechnology, vol. 2. Springer Science Publishers, New York.

  5. Qu J, Mao HZ, Chen W, Gao SQ, Bai YN, Sun YW, Geng YF, Ye J*. 2012. Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. Biotechnology for biofuels 5:10.

  6. Qu J, Ye J, Fang R. 2012. Artificial MicroRNAs for Plant Virus Resistance, Antiviral Resistance in Plants: Methods and Protocols, Methods in Molecular Biology, vol. 894.

  7. Sun F, Liu P, Ye J, Lo LC, Cao S, Li L, Yue GH, Wang CM. 2012. An approach for jatropha improvement using pleiotropic QTLs regulating plant growth and seed yield. Biotechnology for biofuels 5:42.

  8. Gao S, Qu J, Chua NH, Ye J*. 2010. A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. Archives of virology 155:607-612.

  9. Ye J, Qu J, Zhang JF, Geng YF, Fang RX. 2009. A critical domain of the Cucumber mosaic virus 2b protein for RNA silencing suppressor activity. FEBS letters 583:101-106.

  10. Ye J, Qu J, Bui HT, Chua NH. 2009. Rapid analysis of Jatropha curcas gene functions by virus-induced gene silencing. Plant biotechnology journal 7:964-976.

  11. Qu J, Ye J#, Fang R. 2007. Artificial microRNA-mediated virus resistance in plants. Journal of virology 81:6690-6699.

  12. Xu Y, Ye J#, Liu H, Cheng E, Yang Y, Wang W, Zhao M, Zhou D, Liu D, Fang R. 2008. DNA-templated CMV viral capsid proteins assemble into nanotubes. Chemical Communications 1:49-51.

  13. Ye J, Liu L, Xue Y, Qu J, Gao G, Fang R. 2007. Efficient depletion of multiple SARS-CoV mRNAs by a single small interfering RNA targeting the leader sequence. Progress in Biochemistry and Biophysics 34:1092-1100.


国际专利

  1. Jian Ye, et al. Transcriptome and functional analysis reveals a single gene hybrid vigor for oil yield in oil palm. (US Provisional Patent Application No. 62/082,341)

  2. Jian Ye et al.. A method to enhance plant virus resistance. (United States patent, PCT/SG2014/00003)

  3. Jian Ye et al. Control of pests in plants. (U.S. Patent Application US 2012/02400288A1).

  4. Jian Ye et al. Virus induced gene silencing (VIGS) for functional analysis of genes in cotton. (United States Patent 20120096595).

  5. Jian Ye et al. Functional analysis of Jatropha curcas genes. (United States Patent 20110265233).

  6. Jian Ye et al. Genetic Transformation of Jatropha curcas. (United States Patent 20110247099 A1).

  7. Jian Ye et al. Increasing cotton fiber length by manipulating transcription factor. (United States Patent PCT/SG2012/000139).

  8. Jian Ye et al. Microsomal ω6 Oleate Desaturases. (U.S. patent application PCT/SG2011/000337).

 

近期代表工作1双生病毒抑制植物MYC2 介导的茉莉酸(JA)抗虫代谢途径,同介体昆虫烟粉虱形成互惠共生关系。A.植物MYC2转录因子转录激活抗虫次级代谢物合成; B. 双生病毒抑制植物MYC2介导的抗虫反应; C. 双生病毒同烟粉虱形成依赖于寄主植物的互惠共生关系 (Li et al. Plant Cell 2014)

近期代表工作2双生病毒“劫持”细胞质RNA降解途径抑制植物基因沉默并促进病毒复制。Ye et al. PLoS Pathogens 2015

近期代表工作3:棉花曲叶病毒感染增强棉花对棉铃虫的抗性: A. 烟粉虱和棉铃虫在健康棉花和感染棉花曲叶病毒棉花上存活五天后的照片;B. 烟粉虱在健康棉花和感染棉花曲叶病毒棉花上的存活数;C. 棉铃虫在健康棉花、感染棉花曲叶病毒棉花和转基因Bt棉花上体重增长情况。

 

 

 

 

 

 

 

 

80%的植物病毒病害由昆虫媒介传播,1100种已知植物病毒中30%由烟粉虱传播。我们主要研究在自然条件下病毒病害发生的多元生物互作体系的贡献及其互作机制,探求病毒病害防治的重要节点,结合利用大数据平台技术,设计农业生产条件下有效的病毒防治策略。