分子病毒及疫苗研发研究组

组长:邓涛 博士、研究员

研究组研究方向


流感病毒复制机制及抗病毒策略研发



研究组研究内容


1. 流感病毒基因组RNA转录与复制的发生、调控及其结构基础;

2. 流感病毒RNA序列与结构特征参与病毒RNA合成、运输和选择性包装的分子机制;

3. 病毒-宿主相互作用参与病毒RNA合成及其在跨种传播中的作用和机制;

4. 新型流感病毒疫苗及抗流感药物研发。



研究队伍

研究组长:邓涛

电话:64807980

电子邮件:dengt#im.ac.cn(请将#替换成@)

通讯地址:北京市朝阳区北辰西路1号院3号

邮政编码:100101


学习经历

2002.11-2006.03 英国牛津大学病理系 分子病毒学 博士

1988.09-1992.06 兰州大学生物系 生物学 学士


工作经历

2020.03-至今 中国科学院微生物研究所病原室,研究员

2009.10-2020.03 中国医学科学院病原生物学研究所,研究员

2009.01-2009.10 英国国家生物制品标准与控制研究院,资深科学家

2006.04-2008.12 英国牛津大学邓肯病理学院,博士后

2001.11-2006.03 英国牛津大学邓肯病理学院,研究助理

2001.04-2001.11 英国牛津大学邓肯病理学院,访问学者

1992.06-2000.08 中国卫生部兰州生物制品研究所,助理研究员



发表论文


1. Yang R, Pan M, Guo J, Huang Y, Zhang QC*,  Deng T*, Wang J*. 2024. Mapping of the influenza A virus genome RNA structure and interactions reveals essential elements of viral replication.  Cell Rep., 43(3):113833. doi: 10.1016/j.celrep.2024.113833.

2. Zhang L, Shao Y, Wang Y, Yang Q, Guo J, Gao GF,  Deng T*. 2024. Twenty natural amino acid substitution screening at the last residue 121 of influenza A virus NS2 protein reveals the critical role of NS2 in promoting virus genome replication by coordinating with viral polymerase.  J. Virol., 98(1):e0116623. doi: 10.1128/jvi.01166-23.

3. Cao M, Jia Q, Li J, Zhao L, Zhu L, Zhang Y, Li S,  Deng T*. 2024. Naturally occurring PAE206K point mutation in 2009 H1N1 pandemic influenza viruses impairs viral replication at high temperatures.  Virol. Sin., 39(1):71-80. doi: 10.1016/j.virs.2023.11.005.

4. Zhang L, Wang Y, Shao Y, Guo J, Gao GF*,  Deng T*. Fine Regulation of Influenza Virus RNA Transcription and Replication by Stoichiometric Changes in Viral NS1 and NS2 Proteins.  J. Virol., 97(5):e0033723. doi: 10.1128/jvi.00337-23.

5. Shu C, Sun Q, Fan G, Peng K, Yu Z, Luo Y, Gao S, Ma J,  Deng T, Hu S, Wu L. 2024. VarEPS-Influ: an risk evaluation system of occurred and virtual variations of influenza virus genomes.  Nucleic Acids. Res., 52(D1):D798-D807. doi: 10.1093/nar/gkad912.

6. Wang L, Guo J, Wang Y, Zhao P, Liu B, Zhang Y, Xiong Y, Chen Q, Lin L, Li L, He X, Tan Y, Cao M, Yi J,  Deng T*, Lu C*. 2022. Anti-inflammatory effects of Chaishi Tuire Granules on influenza A treatment by mediating TRAF6/MAPK14 axis.  Front. Med. (Lausanne), 9:943681. doi: 10.3389/fmed.2022.943681.

7. Pan M, Zhang W, Xiao Y, Lai Y, Cao M, Wang J,  Deng T*. 2022. The Hierarchical Sequence Requirements of the H1 Subtype-Specific Noncoding Regions of Influenza A Virus.  Microbiol. Spectr., 10(6):e0315322. doi: 10.1128/spectrum.03153-22.

8. Gao P, Ji M, Liu X, Chen X, Liu H, Li S, Jia B, Li C, Ren L, Zhao X, Wang Q, Bi Y, Tan X, Hou B, Zhou X, Tan W,  Deng T, Wang J, Gao GF, Zhang F. 2022. Apolipoprotein E mediates cell resistance to influenza virus infection.  Sci. Adv., 8(38):eabm6668. doi: 10.1126/sciadv.abm6668.

9. Zhao J, Wang J, Pang X, Liu Z, Li Q, Yi D, Zhang Y, Fang X, Zhang T, Zhou R, Zhang T, Guo Z, Liu W, Li X, Liang C,  Deng T, Guo F, Yu L, Cen S. 2022. An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.  Nat. Commun., 13(1):2079. doi: 10.1038/s41467-022-29690-x.

10. Zhou Z, Zhang X, Lei X, Xiao X, Jiao T, Ma R, Dong X, Jiang Q, Wang W, Shi Y, Zheng T, Rao J, Xiang Z, Ren L,  Deng T, Jiang Z, Dou Z, Wei W, Wang J. 2021. Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection.  Signal Transduct Target Ther., 6(1):382. doi: 10.1038/s41392-021-00800-3.

11. Xiao Y, Zhang W, Pan M, Bauer DLV, Bi Y, Cao M, Fodor E,  Deng T*. 2021. Synergistic Effect between 3'-Terminal Noncoding and Adjacent Coding Regions of the Influenza A Virus Hemagglutinin Segment on Template Preference.  J. Virol., 95(18):e0087821. doi: 10.1128/JVI.00878-21.

12. Gao S, Zhang W, Lu C, Cao M, Cen S, Peng Y,  Deng T*.2019. Identification of a type-specific promoter element that differentiates between influenza A and B viruses.  J. Virol., 93(23). doi: 10.1128/JVI.01164-19.

13. Peng Q, Liu Y, Peng R, Wang M, Yang W, Song H, Chen Y, Liu S, Han M, Zhang X, Wang P, Yan J, Zhang B, Qi J,  Deng T, Gao GF, Shi Y. 2019. Structural insight into RNA synthesis by influenza D polymerase.  Nat Microbiol., 4(10):1750-1759. doi: 10.1038/s41564-019-0487-5.

14. Wang J, Zhang Y, Li Q, Zhao J, Yi D, Ding J, Zhao F, Hu S, Zhou J,  Deng T, Li X, Guo F, Liang C, Cen S. 2019. Influenza Virus Exploits an Interferon-Independent lncRNA to Preserve Viral RNA Synthesis through Stabilizing Viral RNA Polymerase PB1.  Cell Rep., 27(11). doi: 10.1016/j.celrep.2019.05.036.

15. Li C, Wang Z, Cao Y, Wang L, Ji J, Chen Z,  Deng T, Jiang T, Cheng G, Qin FX. 2017. Screening for Novel Small-Molecule Inhibitors Targeting the Assembly of Influenza Virus Polymerase Complex by a Bimolecular Luminescence Complementation-Based Reporter System.  J. Virol.,91(5).doi: 10.1128/JVI.02282-16.

16. Wang J, Li J, Zhao L, Cao M,  Deng T*. 2017. Dual Roles of the Hemagglutinin Segment-Specific Noncoding Nucleotides in the Extended Duplex Region of the Influenza A Virus RNA Promoter.  J. Virol., 91(1). doi: 10.1128/JVI.01931-16.

17. Wang Z, Zhao F, Gao Q, Liu Z, Zhang Y, Li X, Li Y, Ma W,  Deng T,Zhang Z, Cen S. 2015. Establishment of a High-Throughput Assay to Monitor Influenza A Virus RNA Transcription and Replication.  PLoS One, 10(7). doi: 10.1371/journal.pone.0133558

18. Wang J, Peng Y, Zhao L, Cao M, Hung T,  Deng T*. 2014. Influenza A virus Utilizes a Suboptimal Kozak Sequence to Fine-tune Virus Replication and Host Response.  J Gen Virol., 96(Pt 4). doi: 10.1099/vir.0.000030.

19. Li C, Wu A, Peng Y, Wang J, Guo Y, Chen Z, Zhang H, Wang Y, Dong J, Wang L, Qin FX, Cheng G,  Deng T*, Jiang T*. 2014. Integrating computational modeling and functional assays to decipher the structure-function relationship of influenza virus PB1 protein.  Sci Rep., 26(4). doi: 10.1038/srep07192.

20. Cao M, Wei C, Zhao L, Wang J, Jia Q, Wang X, Jin Q,  Deng T*. 2014. DnaJA1/Hsp40 is co-opted by influenza A virus to enhance its viral RNA polymerase activity.  J. Virol.,88(24), doi: 10.1128/JVI.02475-14.

21. Zhao L, Peng Y, Zhou K, Cao M, Wang J, Wang X, Jiang T, Deng T*.2014. New insights into the nonconserved noncoding region of the subtype-determinant hemagglutinin and neuraminidase segments of influenza A viruses.  J. Virol.,88(19). doi: 10.1128/JVI.01337-14.

22. Zhou Z, Cao M, Guo Y, Zhao L, Wang J, Jia X, Li J, Wang C, Gabriel G, Xue Q, Yi Y, Cui S, Jin Q*, Wang J*,  Deng T*. 2014. Fragile X mental retardation protein stimulates ribonucleoprotein assembly of Influenza A virus. Nat. Commun., 5:3259. doi: 10.1038/ncomms4259.

23. Tefsen B, Lu G, Zhu Y, Haywood J, Zhao L,  Deng T, Qi J, Gao GF. 2014. The N-terminal domain of PA from bat-derived influenza-like virus H17N10 has endonuclease activity.  J. Virol., 88(4):1935-41. doi: 10.1128/JVI.03270-13.

24. Li C, Ba Q, Wu A, Zhang H,  Deng T*, Jiang T*. 2013. A peptide derived from C-terminus of PB1 inhibits influenza virus replication by interfering with viral polymerase assembly.  FEBS J., 280(4). doi: 10.1111/febs.12107.

25. Dreger M, Leung BW, Brownlee GG*,  Deng T*. 2009. A quantitative proteomics strategy to detect protein regions affected by protein-protein interactions.  Protein Sci., 18(7). doi: 10.1002/pro.159.

26. Kashiwagi T, Leung BW,  Deng T, Chen H, Brownlee GG. 2009. The N-terminal region of the PA subunit of the RNA polymerase of influenza A/HongKong/156/97 (H5N1) influences promoter binding.  PLoS One. 4(5):e5473. doi: 10.1371/journal.pone.0005473.

27. Yuan P, Bartlam M, Lou Z, Chen S, Zhou J, He X, Lv Z, Ge R, Li X,  Deng T, Fodor E, Rao Z, Liu Y. 2009. Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site.  Nature. 458(7240):909-13. doi: 10.1038/nature07720.

28. Chase G#,  Deng T#, Fodor E, Mayer D, Schwemmle M, Brownlee G. 2006. Hsp90 inhibitors reduce influenza virus replication in cell culture.  Virology,377(2). doi: 10.1016/j.virol.2008.04.040.

29.Deng T#, Sharps J, Brownlee GG. 2006. The role of the influenza virus heterotrimeric RNA polymerase complex in the initiation of replication.  J Gen Virol., 87(Pt11): 3373-3377.

30.Deng T#,Engelhardt OG*, Thomas B, Thomas B, Akoulitchev AV, Brownlee GG, Fodor E. 2006. The role of Ran binding protein 5 (RanBP5) in the nuclear import and assembly of he influenza virus RNA polymerase complex.  J. Virol., 80:11911-11919.

31.Deng T#, Vreede FT#, Brownlee GG. 2006. Different de novo initiation strategies are used by influenza virus RNA polymerase on its cRNA and viral RNA promoters during viral RNA replication.  J. Virol.,80(5):2337-2348.

32.Deng T#, Sharps J, Fodor E, Brownlee GG. 2005. In vitro assembly of PB2 with PB1-PA dimer supports a new model assembly of influenza A virus polymerase subunits into a functional trimeric complex.  J. Virol., 79(13):8669-8674.

33. Crow M#,  Deng T#,Addley M, Brownlee GG. 2004. Mutational analysis of the influenza virus cRNA promoter and identification of nucleotides critical for replication.  J. Virol., 78(12):6263-6270.

34. Fodor E, Mingay LJ, Crow M,  Deng T, Brownlee GG. 2003. A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase promotes the generation of defective interfering RNAs. J. Virol., 77(8):5017-5020.

35. Fodor E, Crow M, Mingay LJ,  Deng T, Sharps J, Fechter P, Brownlee GG. 2002. A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase inhibits endonucleolytic cleavage of capped RNAs.  J. Virol., 76(18):8989-9001.


已授权国家发明专利


1.    邓涛、郭阳、周卓。一种提高流感病毒疫苗株产量的方法,专利授权号ZL 2012 1 0299497.2, 授权日期:2015.08.05 

2.    邓涛,赵丽丽。甲型流感病毒亚型基因型定型的方法,专利授权号ZL 2014 1 0354092.3 授权日期:2017.01.18