微生物分子生理学与代谢工程研究组

 组长：张延平 博士、研究员

 研究组研究方向

     合成生物学与系统代谢工程

 研究组研究内容及意义

 研究组长

研究组长：张延平 博士 研究员

电话：010-64807351

电子邮件：zhangyp#im.ac.cn（请将#替换成@）

通讯地址：北京市朝阳区北辰西路1号院3号，中国科学院微生物研究所

邮政编码：100101

 主要学习及工作经历

1996.9-2000.7 清华大学化学工程系，化学工程与工艺，学士学位

2000.9-2006.7 清华大学化学工程系，化学工程与技术，博士学位

2006.07-2010.07 中国科学院微生物研究所，助理研究员

2010.07-2016.09 中国科学院微生物研究所，副研究员

2016.10-2022.10 中国科学院微生物研究所，项目研究员

2022.12-至今，  中国科学院微生物研究所，研究员

 研究团队

    1. Huawei Zhu, Liru Xu, Guodong Luan, Tao Zhan, Zepeng Kang, Chunli Li, Xuefeng Lu*, Xueli Zhang* , Zhiguang Zhu*, Yanping Zhang* & Yin Li*. A miniaturized bionic ocean-battery mimicking the structure of marine microbial ecosystems.Nature Communications.2022, 13: 5608

    2. Lu Xiao, Guoxia Liu, Fuyu Gong, Huawei Zhu, Yanping Zhang, Zhen Cai, and Yin Li*. A minimized synthetic carbon fixation cycle.ACS Catalysis. 2022, 12, 799-808

    3. Huawei Zhu, Yin Li*. Turning waste gases into valuables.Synthetic and Systems Biotechnology. 2022, 7(3):847-848

    4. Guoxia Liu, Florence Hui-Ping Tan, Sie-Yik Amy Lau, Mohamad Hafis Jaafar, Fiona Yi-Li Chung, Ghows Azzam*, Min-Tze Liong*, Yin Li*. Lactic acid bacteria feeding reversed the malformed eye structures and ameliorated gut microbiota profiles ofDrosophila melanogasterAlzheimer’s Disease model.Journal of Applied Microbiology.2022, 132: 3155-3167

    2021年

    1. Zongjie Dai^#, Yan Zhu^#, Hongjun Dong, Chunhua Zhao, Yanping Zhang*, Yin Li. Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production inClostridium acetobutylicum. Microbial Cell Factories.2021, 20(1): 149

    2. Tongxing Zhao, Yanping Zhang*, Yin Li*. Biological carbon fixation: a thermodynamic perspective.Green Chemistry.2021, 23: 7852-7864

    3. Hengkai Meng, Wei Zhang, Huawei Zhu, Fan Yang, Yanping Zhang, Jie Zhou* and Yin Li*. Over-expression of an electron transport protein OmcS provides suffcient NADH for d-lactate production in cyanobacterium.Biotechnology for Biofuels.2021, 14: 109

    4. Lu Xiao, Guoxia Liu, Fuyu Gong, Zhen Cai, Yin Li*. The reductive carboxylation activity of heterotetrameric pyruvate synthases from hyperthermophilic archaea.Biochemical and Biophysical Research Communications.2021, 572: 151-156

    5. Tongxin Zhao, Liru Xu, Lei Zhao, Hongjie Zhang, Yin Li*, Yanping Zhang*. BtsT/ BtsS is involved in glyoxylate transport inE. coliand its mutations facilitated glyoxylate utilization.Biochemical and Biophysical Research Communications.2021, 551: 71-77

    6. Junli Zhang^#, Guoxia Liu^#, Alonso I. Carvajal, Robert H. Wilson*, Zhen Cai*, Yin Li*. Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO₂capture.Bioresources and Bioprocessing.2021, 8: 86

    7. Jean Paul Sinumvayo, Yin Li*, Yanping Zhang*. Microbial production of butyl butyrate: from single strain to cognate consortium.Bioresources and Bioprocessing.2021, 8: 50

    8. Jean Paul Sinumvayo, Chunhua Zhao, Guoxia Liu, Yanping Zhang*, Yin Li*.One-pot production of butyl butyrate from glucose by constructing a “diamond-shaped”E. coliconsortium.Bioresources and Bioprocessing.2021, 8: 18

    9. Jie Zhou, Fan Yang, Fuliang Zhang, Hengkai Meng, Yanping Zhang, and Yin Li*. Impairing photorespiration increases photosynthetic conversion of CO₂to isoprene in engineered cyanobacteria.Bioresources and Bioprocessing.2021, 8: 42

    10. Fan Yang, Junli Zhang, Zhen Cai, Jie Zhou* and Yin Li*. Exploring the oxygenase function of Form II Rubisco for production of glycolate from CO₂.AMB Express.2021, 11: 65

    11. Guoxia Liu, Nooreen Mamode Ally, Daminee Devi Dooly, Yin Li, Kamleshwar Boodhoob, Hudaa Neetoob*. A study on the effectiveness of a defined microbial consortium to enhance the microbiological safety of cattle manure.Journal of the Science of Food and Agriculture.2021,101(6): 2614-2620

    2020年

    1. Taicheng Zhu, Tongxin Zhao, Bankefa Olufemi, Yin Li *. Engineering unnatural methylotrophic cell factories for methanol-based biomanufacturing: challenges and opportunities.Biotechnology Advances.2020, 39: 107467

    2. Chunhua Zhao, Yanping Zhang*, Yin Li*. Metabolic engineering for the production of butanol, a potential advanced biofuel, from renewable resources.Biochemical Society Transactions.2020, 48: 2283-2293

    3. Sijie Yu, Liangtian Miao, He Huang*, Yin Li, Taicheng Zhu*. High-level production of glucose oxidase inPichia pastoris: effects of Hac1p overexpression on cell physiology and enzyme expression.Enzyme and Microbial Technology.2020, 141: 109671

    4. Guoxia Liu, Hui-Xian Chong, Fiona Yi-Li Chung, Yin Li*, Min-Tze Liong*.Lactobacillus plantarumDR7 modulated bowel movement and gut microbiota associated with dopamine and serotonin pathways in stressed adults. InternationalJournal of Molecular Sciences.2020, 21: 4608

    5. Florence Hui Ping Tan#, Guoxia Liu#, Sie-Yik Amy Lau, Mohamad Hafis Jaafar, Yong-Ha Park, G Azzam*, Yin Li*, Min-Tze Liong*.Lactobacillusprobiotics improved the gut microbiota profile of aDrosophila melanogasterAlzheimer’s disease model and alleviated neurodegeneration in the eye.Beneficial Microbes.2020, 11(1): 79-89

    2019年

    1. Huawei Zhu, Hengkai Meng, Wei Zhang, Haichun Gao, Jie Zhou, Yanping Zhang*, Yin Li*. Development of a longevous two-species biophotovoltaics with constrained electron flow.Nature Communications.2019, 10: 4282

    2. Chunhua Zhao^#, Jean Paul Sinumvayo^#, Yanping Zhang*, Yin Li*. Design and development of a “Y-shaped” microbial consortium capable of simultaneously utilizing biomass sugars for efficient production of butanol.Metabolic Engineering.2019, 55: 111-119

    3. Chunhua Zhao, Hongjun Dong, Yanping Zhang*, Yin Li*.Discovery of potential genes contributing to the biosynthesis of short-chain fatty acids and lactate in gut microbiota from systematic investigation inE. coli.npj Biofilms and Microbiomes.2019, 5: 19

    4. Chunhua Zhao, Yanping Zhang*, Yin Li*. Production of fuels and chemicals from renewable resources using engineeredEscherichia coli.Biotechnology Advances.2019, 37: 107402

    5. Taicheng Zhu, Hongbing Sun, Meiyu Wang, Yin Li*.Pichia pastorisas a versatile cell factory for the production of industrial enzymes and chemicals: current status and future perspectives.Biotechnology Journal.2019, 14 (6): e1800694

    6. Yuwan Liu#, Xiaoyun Lu#, Yiqun Yang#, Shanshan Wang#, Qian Wang, Xiya Wang, Zhihui Yan, Jian Cheng, Cui Liu, Xue Yang, Hao Luo, Sheng Yang, Junran Gou, Luzhen Ye, Lina Lu, Zhidan Zhang, Yu Guo, Yan Nie, Jianping Lin, Sheng Li, Chaoguang Tian, Wen Wang, Hongwu Ma, Yongjun Liu*, Yin Li*, Huifeng Jiang*, Yanhe Ma. Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design.Nature Communications.2019, 10: 1378

    7. Jie Zhou, Yin Li*. SNPs deciding the rapid growth of cyanobacteria are alterable.Proceeding of the National Academy of Sciences of the United States of American.2019, 116 (10): 3945

    8. Fuyu Gong, Huawei Zhu, Jie Zhou, Tongxin Zhao, Lu Xiao, Yanping Zhang*, Yin Li*. An Economy Based on Carbon Dioxide and Water: Enhanced biological fixation of CO₂using microorganisms. (book).2019, https://doi.org/10.1007/978-3-030-15868-2

   2018年

    1. Guipeng Hu#, Jie Zhou#, Xiulai Chen, Yuanyuan Qian, Cong Gao, Liang Guo, Peng Xu, Wei Chena, Jian Chen, Yin Li, Liming Liu*. Engineering synergetic CO₂-fixing pathways for malate production.Metabolic Engineering.2018, 47: 496-504

    2. Fitsum Tigu, Junli Zhang, Guoxia Liu, Zhen Cai*, Yin Li*. A highly active pantothenate synthetase fromCorynebacterium glutamicumenables the production ofd-pantothenic acid with high productivity.Applied Microbiology and Biotechnology.2018, 102(14): 6039-6046

    3. Olufemi Emmanuel Benkefa, Meiyu Wang, Taicheng Zhu*, Yin Li*. Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the methylotrophic yeastPichia pastoris.Bioresources and Bioprocessing.2018, 5(1): 25

    4. Fuyu Gong, Huawei Zhu, YanpingZhang*, Yin Li*. Biological carbon fixation: from natural to synthetic.Journal of CO₂Utilization.2018, 28: 221-227

    5. Olufemi Emmanuel Benkefa, Meiyu Wang, Taicheng Zhu*, Yin Li*. Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeastPichia pastoris.Biotechnology Letters.2018, 40(7): 1149-1156

    6. Jie Zhou, Hengkai Meng, Wei Zhang, Yin Li*. Production of industrial chemicals from CO₂by engineering Cyanobacteria.Advances in Experimental Medicine and Biology.2018, 1080: 97-116

已授权专利

1. 赵春华，张延平，李寅，Jean Paul Sinumvayo，张天瑞，董红军.利用木糖生产丁醇的工程菌株及其构建方法和应用.201910299026.3

2.   蔡真，张君丽，奇古，王瑞研，李寅.一种制备高效合成泛酸基因工程菌的方法及其应用.201710038544.0

3.   朱泰承，孙红兵，李寅.高效耐受和利用甲醇的酵母菌株及应用.201610134833.6

4.   董红军，赵春华，张延平，李寅.一种提高大肠杆菌生产丁醇的方法.201610035150.5

5.   赵春华，董红军，张延平，李寅.一种丁酸产量低且丁醇产量高的工程菌及其构建方法与应用.201610034891.1

6.   董红军，赵春华，林兆，张延平，李寅.一种构建高产丁醇菌株的方法与应用.201510924308.x

7.  蔡真，张君丽，李寅，张冬竹，郭恒华.用于生产β-丙氨酸的工程菌及生产β-丙氨酸的方法. 201510067784.4，PCT/CN2015/074683

8.    朱泰承，孙红兵，李鹏飞，李寅，邓启华，沈建国，陆英，林智平，贾凤超.一种高效分泌表达纳豆激酶的重组菌.201410109952.7

9.   蔡真，张君丽，邓思颖，李寅.一种高L-天冬氨酸α羧化酶活性的工程菌及其在生产β-丙氨酸中的应用.201310534782.2

10.  马延和，朱泰承，孙红兵，李鹏飞，薛燕芬，李寅.一种组成型表达碱性β-甘露聚糖酶的重组菌.201310120494.2

11.  蔡真，李兴华，张延平，李寅. 1,2,4-丁三醇的生物合成方法.201210457975.8

12.  张延平，张博，张海峰，孙红兵，李寅，马延和.一种酿酒酵母工程菌及其在生产乙醇中的应用.201210180994.0

13.   董红军，戴宗杰，张延平，李寅.一种稳定联产异丙醇和丁醇的工程菌及其构建方法与应用. 201110084263.1