蛋白质工程与微生物酶应用研究组

 组长:唐双焱 博士、研究员

  

 

 研究组研究方向

 

  代谢工程、蛋白质工程、合成生物学 

 

  研究组研究内容及意义

  

  1.碳水化合物酶的改造

  我们采用蛋白质工程手段(定向进化以及理性设计法)对微生物酶进行改造,创造或提升酶的各方面优良特性,以扩大其应用价值。我们主要进行碳水化合物酶类如糖苷酶、糖基转移酶的改造研究,对该类酶的底物或产物特异性、活性、稳定性等进行改造以提升它们的应用价值。

 

  2.小分子化合物的生物合成

  我们在大肠杆菌中对高价值小分子化合物(药物、食品添加剂以及其他化合物)的生物合成途径进行改造。该研究方向旨在将自然界大量存在的廉价资源用微生物高效转化为自然界不存在或含量很低的一些高价值小分子化合物。我们主要采用定向进化方法对这些化合物的生物合成途径及宿主细胞进行改造,以提高化合物的生物合成效率。

 

   

 研究组长

 

  研究组长:唐双焱 博士 研究员

  电话:010-64806140

  电子邮件:tangsy#im.ac.cn(请将#换为@

  通讯地址:北京市朝阳区北辰西路1号院3号,中国科学院微生物研究所

  邮政编码:100101

 

 

 主要学习及工作经历

    

  学习经历

  1991年-1995年,武汉大学,获得生物化学学士学位。

  1995年-1998年,武汉大学,获得生物化学硕士学位。

  2003年-2006年,韩国首尔大学,获得食品科学博士学位

  工作经历

  1998年至2003年,云南省药品检验所

  2006年至2010年,美国宾夕法尼亚州立大学,博士后

  2011年至今,中国科学院微生物研究所,研究员

 

 

 研究团队

 

  

  

 工作人员   

  陈 伟 博士 副研究员

  梁朝宁 博士 助理研究员

  吴结缘 硕士 助理研究员

 

 在读研究生   

  姚 骏 直博生(2014-)

  张旋旋 直博生(2015-)

  李世忠 博士生(2017-)

  雷严严 直博生(2017-)

  曹玉峰 直博生(2019-)

  侯 杰 硕士生(2017-)

  朱小冲 硕士生(2018-)

  甄 珍 硕士生(2018-)

  彭冰冰 硕士生(2019-)

 

 已毕业学生

  张轶,穆姗姗,熊丹丹,朱琳,陆仕坤,李标,李静,李恒,孟杰

  

 

 代表性论文

 

  1. Jun Yao#, Yang He#, Nannan Su, Sakshibeedu R. Bharath, Yong Tao, Jian-Ming Jin*, Wei Chen*, Haiwei Song*, Shuang-Yan Tang*. Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps. Nature Communications 2020, accepted 

  2. Jun Yao#, Jing Li#, Dandan Xiong, Yuanyuan Qiu, Guizhi Shi, Jian-Ming Jin*, Yong Tao*, Shuang-Yan Tang*. Development of a highly efficient and specific l-theanine synthase. Applied Microbiology and Biotechnology 2020, https://doi.org/10.1007/s00253-020-10482-6 

  3. Chaoning Liang, Xuanxuan Zhang, Jieyuan Wu, Shanshan Mu, Zhe Wu, Jian-Ming Jin*, Shuang-Yan Tang*, Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit. Metabolic Engineering 2020, 57: 239-246 

  4. Wei Chen#, Jun Yao#, Jie Meng, Wenjing Han, Yong Tao, Yihua Chen, Yixin Guo, Guizhi Shi, Yang He*, Jian-Ming Jin*, Shuang-Yan Tang*, Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis. Nature Communications 2019, 10: 960. 

  5. Wei Chen#, Xuanxuan Zhang#, Dandan Xiong#, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues. Applied Microbiology and Biotechnology 2019, 103: 3205-3213. 

  6. Heng Li, Jing Li, Ruinan Jin, Wei Chen, Chaoning Liang, Jieyuan Wu, Jian-Ming Jin, Shuang-Yan Tang*, Towards the construction of high-quality mutagenesis libraries. Biotechnology Letters 2018, 40: 1101-1107. 

  7. Heng Li, Wei Chen, Ruinan Jin, Jian-Ming Jin*, Shuang-Yan Tang*, Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products. Microbial Cell Factories 2017, 16: 187. 

  8. Heng Li, Chaoning Liang, Wei Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Yong Tao, Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid. Biosensors and Bioelectronics 2017, 98: 457-465.  

  9. Jieyuan Wu, Peixia Jiang, Wei Chen, Dandan Xiong, Linglan Huang, Junying Jia, Yuanyuan Chen, Jian-Ming Jin*, Shuang-Yan Tang*, Design and application of a lactulose biosensor. Scientific Reports 2017, 7: 45994. 

   10. Qingzhuo Wang, Shuang-Yan Tang*, Sheng Yang*. Genetic biosensors for small-molecule products: Design and applications in high-throughput screening. Frontiers of Chemical Science and Engineering 2017, 11: 15-26.

  11. Dandan Xiong, Shikun Lu, Jieyuan Wu, Chaoning Liang, Wei Wang, Wenzhao Wang, Jian-Ming Jin*, Shuang-Yan Tang*, Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor. Metabolic Engineering 2017, 40: 115-123. 

  12. Lin Zhu#, Zhe Wu#, Jian-Ming Jin, Shuang-Yan Tang*, Directed evolution of leucine dehydrogenase for improved efficiency of l-tert-leucine synthesis. Applied Microbiology and Biotechnology 2016, 100: 5805-5813. 

  13. Chaoning Liang#, Yi Zhang#, Yan Jia, Wenzhao Wang, Youhai Li, Shikun Lu, Jian-Ming Jin*, Shuang-Yan Tang*. Engineering a carbohydrate-processing transglycosidase into glycosyltransferase for natural product glycodiversification. Scientific Reports 2016, 6: 21051.  

  14. Wei Chen#, Shan Zhang#, Peixia Jiang#, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong*, Shuang-Yan Tang*. Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis. Metabolic Engineering 2015, 30:149-155. 

  15. Peixia Jiang#, Shanshan Mu#, Heng Li, Youhai Li, Congmin Feng, Jian-Ming Jin*, Shuang-Yan Tang*. Design and application of a novel high-throughput screening technique for 1-deoxynojirimycin. Scientific Reports 2015, 5: 8563. 

  16. Chaoning Liang#, Xiwu Gui#, Cheng Zhou, Yanfen Xue, Yanhe Ma*, Shuang-Yan Tang*. Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming. Applied Microbiology and Biotechnology 2015, 99: 2673-2682. 

  17. Chaoning Liang#, Dandan Xiong#, Yi Zhang, Shanshan Mu, Shuang-Yan Tang*. Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology 2015, 99:2267-2275. 

  18. Shuang-Yan Tang, Shuai Qian, Olubolaji Akinterinwa, Christopher S. Frei, Joseph A. Gredell, Patrick C. Cirino. Screening for enhanced triacetic acid lactone production by recombinant Escherichia coli expressing a designed triacetic acid lactone reporter. Journal of the American Chemical Society 2013, 135 (27): 10099-10103.  

  19. Shuang-Yan Tang, Patrick C. Cirino. Design and application of a mevalonate- responsive regulatory protein. Angewandte Chemie International Edition 2011, 50: 1084-1086. 

  20. Shuang-Yan Tang, Patrick C. Cirino. Elucidating residue roles in engineered variants of AraC regulatory protein. Protein Science 2010, 19: 291-298. 

  21. Shuang-Yan Tang, Hossein Fazelinia, Patrick C. Cirino. AraC regulatory protein   mutants with altered effector specificity. Journal of the American Chemical Society  2008, 130: 5267-5271.  

  22. Shuang-Yan Tang, Sung-Jae Yang, Hyunju Cha, Eui-Jeon Woo, Cheonseok Park, Kwan-Hwa Park. Contribution of W229 to the transglycosylation activity of 4-α- glucanotransferase from Pyrococcus furiosus. Biochimica et Biophysica Acta 2006, 1764: 1633-1638.  

  23. Shuang-Yan Tang, Quang-Tri Le, Jae-Hoon Shim, Sung-Jae Yang, Joong-Huck Auh,  Cheonseok Park, Kwan-Hwa Park. Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling. FEBS Journal, 2006, 273: 3335-3345.

  24. Kyung-Ah Cheong,# Shuang-Yan Tang, # Tae-Kyu Cheong, Hyunju Cha, Jung-Wan Kim, Kwan-Hwa Park. Thermostable and alkalophilic maltogenic amylase of Bacillus thermoalkalophilus ET2 in monomer-dimer equilibrium. Biocatalysis and Biotransformation, 2005, 23: 79-/87  # Contributed equally 

  25. Dan Li, Sung-Hoon Park, Jae-Hoon Shim, Hee-Seob Lee, Shuang-Yan Tang, Cheon- Seok Park and Kwan-Hwa Park. In vitro enzymatic modification of puerarin to puerarin glycosides by maltogenic amylase. Carbohydrate Research 2004, 339: 2789-2797.  

  26. 李恒, 唐双焱*. 碳水化合物结合结构域研究进展[J]. 微生物学报, 2017, 57(8): 1160-1167. 

  27. 唐双焱*梁朝宁,蒋培霞. 高通量筛选工具的设计与应用 生物工程学报,2012, 28(7): 781-788.