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郭惠珊研究组

RNA沉默和植物抗病机制 研究组

组长:郭惠珊 博士 研究员 博士生导师

研究组研究方向

1、植物RNA沉默的分子机制及其抗病应用研究; 2、植物与真菌的互作机理及维管束真菌病害的防控策略研究

研究组研究内容和意义

本研究组以模式植物拟南芥、烟草和重要经济作物棉花为寄主材料,以病毒和真菌为病原微生物,围绕植物RNA沉默和miRNA调控途径,以及其他受病原影响的调控途径开展病原微生物-寄主的相互作用研究,剖析植物致病机理。为植物抗病和病害防控提供有效策略。

研究组长

郭惠珊 博士 研究员
电话及传真:010-64847989
电子邮件:guohs#im.ac.cn(请将#换成@
通讯地址:北京市朝阳区北辰西路1号院3号,
中国科学院微生物研究所
邮编:100101

学习经历
1983-1987,中山大学生物学,学士
1993-1996,西班牙马德里大学,博士

工作经历
1988-1991 中山大学生物工程中心,助教
1992-1993 西班牙马德里大学"Severo Ochoa"分子生物学中心,Guest Investigator
1997-1998 西班牙马德里大学国家生物工程中心(CSIC)博士后
1998-2003 新加坡国立大学分子农业生物学院/淡马锡生命科学研究院Research Fellow
2003-2004 新加坡国立大学淡马锡生命科学研究院,Acting PI
2004-至今 中国科学院"百人计划",中国科学院微生物所 研究员、博士生导师

获奖情况
2005 国家杰出青年科学基金
2008 所长奖教金
2010 所长奖教金
2012 朱李月华优秀教师奖
2015 国务院政府特殊津贴

学术兼职
中国科学院微生物研究所第一届研究组长委员会 委员 2014-
中国科学院微生物研究所学术道德与权益委员会 委员 2014-
中国科学院微生物研究所第八届学位评定委员会 2014-
中国科学院微生物研究所第九届学术委员会 委员 2013-2018
植物基因组学国家重点实验室第三届学术委员会 委员 2011-2015
海南热带生物资源可持续利用重点实验室第一届学术委员会 委员 2011-2015
中国植物病理学会第十届理事会 理事 2014-2018
MPMI Associate Editor 2013-2015
PLoS Pathogens Guest Editor 2013-2014
PLoS Pathogens Associate Editor 2014-
BMC Genomics Associate Editor 2014-

研究队伍(请将#换成@

工作人员
华辰雷 副研究员 huacl#im.ac.cn
金 芸 助理研究员 jiny#im.ac.cn
赵建华 助理研究员 zhao_jian_hua#hotmail.com
房媛媛 助理研究员 fangyy#im.ac.cn

在读学生

姓名 性别 邮箱
赵云龙 zyl714#126.com
张 涛 zhtlyy#163.com
汪晶晶 jingjing20070331#163.com
刘晓兰 lyueyawanwan#163.com
周婷婷 zhouttcsc#hotmail.com
倪 浩 aniger1#aliyun.com
陈中祺 chenzhongqi2006#163.com
李 洁 lijiemuzi2012#163.com
王 胜 shuyeandy#126.com
杜 旋 1511606573#qq.com
张燕玲 zyling116#163.com
马晓薇 mxwwillbeokay#sina.com

已培养学生博士后

类别 姓名 毕业年份
博士 段成国 2008
博士 杜全生 2008
博士 何祥凤 2009
博士 应晓宝 2010
硕士 李 焱 2010
博士 朱 慧 2011
博士 周邦军 2012
博士 徐 宁 2012
博士 房媛媛 2013
博士 冯 镭 2013
博士 赵 盼 2014
博士 赵建华 2015
博士后 杨丽萍 2012

代表性论文

 

Wang, J.J., and Guo, H.S. (2015). Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by microRNA847 upregulates auxin signaling to modulate cell proliferation and lateral organ growth in Arabidopsis. The Plant cell 27, 574-590.

Zahid, K., Zhao, J.H., Smith, N.A., Schumann, U., Fang, Y.Y., Dennis, E.S., Zhang, R., Guo, H.S., and Wang, M.B. (2015). Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA. PLoS genetics 11, e1004906.

Jin, Y., and Guo H.S., (2015). Transgene-induced gene silencing in plants. Methods in Molecular Biology, 2015; 1287: 105-17.

Fang, Y.Y., Smith, N.A., Zhao, J.H., Lee, J.R., Guo, H.S., and Wang, M.B. (2015). Cloning and profiling of small RNAs from cucumber mosaic virus satellite RNA. Methods in molecular biology 1236, 99-109.

Hua, C., Yang, X., and Wang, Y. (In Press). Phytophthora sojae and soybean isoflavones, a model to study zoospore chemotaxis. Physiological and Molecular Plant Pathology.

Zhao, P., Zhao, Y.L., Jin, Y., Zhang, T., and Guo, H.S. (2014). Colonization process of Arabidopsis thaliana roots by a green fluorescent protein-tagged isolate of Verticillium dahliae. Protein & cell 5, 94-98.

Feng, L., Duan, C.G., and Guo, H.S. (2013). Inhibition of in vivo Slicer activity of Argonaute protein 1 by the viral 2b protein independent of its dsRNA-binding function. Molecular plant pathology 14, 617-622.

Yang, L.P., Fang, Y.Y., An, C.P., Dong, L., Zhang, Z.H., Chen, H., Xie, Q., and Guo, H.S. (2013). C2-mediated decrease in DNA methylation, accumulation of siRNAs, and increase in expression for genes involved in defense pathways in plants infected with beet severe curly top virus. The Plant journal : for cell and molecular biology 73, 910-917.

Duan, C.G., Fang, Y.Y., Zhou, B.J., Zhao, J.H., Hou, W.N., Zhu, H., Ding, S.W., and Guo, H.S. (2012a). Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein. The Plant cell 24, 259-274.

Duan, C.G., Wang, C.H., and Guo, H.S. (2012b). Application of RNA silencing to plant disease resistance. Silence 3, 5.

Xu, N., Li, Y., Zhao, Y.T., Guo, L., Fang, Y.Y., Zhao, J.H., Wang, X.J., Huang, L., and Guo, H.S. (2012). Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. PloS one 7, e35306.

Zhou, B.J., Jia, P.S., Gao, F., and Guo, H.S. (2012). Molecular characterization and functional analysis of a necrosis- and ethylene-inducing, protein-encoding gene family from Verticillium dahliae. Molecular plant-microbe interactions : MPMI 25, 964-975.

Zhu, H., and Guo, H. (2012). The role of virus-derived small interfering RNAs in RNA silencing in plants. Science China. Life sciences 55, 119-125.

Dong, L., Liu, M., Fang, Y.Y., Zhao, J.H., He, X.F., Ying, X.B., Zhang, Y.Y., Xie, Q., Chua, N.H., and Guo, H.S. (2011). DRD1-Pol V-dependent self-silencing of an exogenous silencer restricts the non-cell autonomous silencing of an endogenous target gene. The Plant journal : for cell and molecular biology 68, 633-645.

Hou, W.N., Duan, C.G., Fang, R.X., Zhou, X.Y., and Guo, H.S. (2011). Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection. Molecular plant pathology 12, 595-605.

Zhang, Z., Chen, H., Huang, X., Xia, R., Zhao, Q., Lai, J., Teng, K., Li, Y., Liang, L., Du, Q., Zhou, X., Guo, H., and Xie, Q. (2011). BSCTV C2 attenuates the degradation of SAMDC1 to suppress DNA methylation-mediated gene silencing in Arabidopsis. The Plant cell 23, 273-288.

Zhu, H., Duan, C.G., Hou, W.N., Du, Q.S., Lv, D.Q., Fang, R.X., and Guo, H.S. (2011). Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. Journal of virology 85, 13384-13397.

Chen, H., Zhang, Z., Teng, K., Lai, J., Zhang, Y., Huang, Y., Li, Y., Liang, L., Wang, Y., Chu, C., Guo, H., and Xie, Q. (2010). Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway. The Plant journal : for cell and molecular biology 62, 12-23.

Gao, F., Zhou, B.J., Li, G.Y., Jia, P.S., Li, H., Zhao, Y.L., Zhao, P., Xia, G.X., and Guo, H.S. (2010). A glutamic acid-rich protein identified in Verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. PloS one 5, e15319.

Li, Y., Liu, X., Huang, L., Guo, H., and Wang, X.J. (2010). Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea. Journal of genetics and genomics = Yi chuan xue bao 37, 493-503.

Liu, L., Zhang, Y., Tang, S., Zhao, Q., Zhang, Z., Zhang, H., Dong, L., Guo, H., and Xie, Q. (2010). An efficient system to detect protein ubiquitination by agroinfiltration in Nicotiana benthamiana. The Plant journal : for cell and molecular biology 61, 893-903.

Teng, K., Chen, H., Lai, J., Zhang, Z., Fang, Y., Xia, R., Zhou, X., Guo, H., and Xie, Q. (2010). Involvement of C4 protein of beet severe curly top virus (family Geminiviridae) in virus movement. PloS one 5, e11280.

Wang, W., Wu, Y., Li, Y., Xie, J., Zhang, Z., Deng, Z., Zhang, Y., Yang, C., Lai, J., Zhang, H., Bao, H., Tang, S., Yang, C., Gao, P., Xia, G., Guo, H., and Xie, Q. (2010). A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes. Plant molecular biology 72, 91-99.

Ying, X.B., Dong, L., Zhu, H., Duan, C.G., Du, Q.S., Lv, D.Q., Fang, Y.Y., Garcia, J.A., Fang, R.X., and Guo, H.S. (2010). RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in Nicotiana benthamiana. The Plant cell 22, 1358-1372.

Lai, J., Chen, H., Teng, K., Zhao, Q., Zhang, Z., Li, Y., Liang, L., Xia, R., Wu, Y., Guo, H., and Xie, Q. (2009). RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. The Plant journal : for cell and molecular biology 57, 905-917.

Zheng, N., Xia, R., Yang, C., Yin, B., Li, Y., Duan, C., Liang, L., Guo, H., and Xie, Q. (2009). Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. Vaccine 27, 5001-5007.

Duan, C.G., Wang, C.H., Fang, R.X., and Guo, H.S. (2008). Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. Journal of virology 82, 11084-11095.

He, X.F., Fang, Y.Y., Feng, L., and Guo, H.S. (2008). Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica. FEBS letters 582, 2445-2452.

Zhang, Y.Y., Li, Y., Gao, T., Zhu, H., Wang, D.J., Zhang, H.W., Ning, Y.S., Liu, L.J., Wu, Y.R., Chu, C.C., Guo, H.S., and Xie, Q. (2008). Arabidopsis SDIR1 enhances drought tolerance in crop plants. Bioscience, biotechnology, and biochemistry 72, 2251-2254.

Du, Q.S., Duan, C.G., Zhang, Z.H., Fang, Y.Y., Fang, R.X., Xie, Q., and Guo, H.S. (2007). DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. Journal of virology 81, 9142-9151.

Zhang, Y., Yang, C., Li, Y., Zheng, N., Chen, H., Zhao, Q., Gao, T., Guo, H., and Xie, Q. (2007). SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis. The Plant cell 19, 1912-1929.

Xie, Q., and Guo, H.S. (2006). Systemic antiviral silencing in plants. Virus research 118, 1-6.

Guo, H.S., Xie, Q., Fei, J.F., and Chua, N.H. (2005). MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development. The Plant cell 17, 1376-1386.


代表性图片


Plants Overexpressing the IAA28 mRNA Display the Opposite Phenotype to That of 35S-miR159847. (A) The expression of IAA28 mRNA in Col-0, 35S-IAA28 transgenic, and iaa28-ko mutant plants at 33 DAG was quantified by qRT-PCR. (B) Average fresh weight of rosette leaves of Col-0, 35S-miR159847, iaa28-ko, and 35S-IAA28 transgenic plants at 33 DAG with inflorescences removed. (C) Plants with inflorescences removed rosette leaves of 35S-IAA28-1, Col-0, 35S-miR159847-25, and iaa28-ko at 33 DAG. Bars = 10 mm.
Link: http://www.plantcell.org/content/27/3/574.long



Confocal microscopy images of the advanced stage root colonization by V592-GFP1 on Arabidopsis roots. Protein Cell. 2014 Feb; 5(2): 94–98. doi:  10.1007/s13238-013-0009-9

 

 
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